Hearing is a matter of translating the movement of air molecules in a signal to the brain.
There it is processed.
Basically we do have a build in mixer. It mixes the sound and all our cognitions into a perception. Hence if we believe something do makes a difference, we will hear it.
If it is about sound quality, you don’t want to have any interference.
You want to judge the sound and the sound alone.
This is obtained by removing all our cognitions  from the test.
This can be done by blind testing.
In case of hardware this is hard to obtain.
In case of software there are programs available, most of the time ABX testing.
More details about our perception can be found here.


Download the ABX Comparator plug-in.


ABX/Shootout-er blind testing audio software